Plasmid_Backbone

Part:BBa_K2100082

Designed by: Archis R. Bhandarkar   Group: iGEM16_MIT   (2016-10-21)


miRNA Sensor Backbone

This is the common plasmid backbone for all the miRNA sensors used in our project. They contain both ampicillin and kanamycin resistance marker.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 4162
    Illegal XbaI site found at 4168
    Illegal SpeI site found at 1107
    Illegal PstI site found at 4147
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 4162
    Illegal SpeI site found at 1107
    Illegal PstI site found at 4147
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 4162
    Illegal BglII site found at 3690
    Illegal XhoI site found at 2938
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 4162
    Illegal XbaI site found at 4168
    Illegal SpeI site found at 1107
    Illegal PstI site found at 4147
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2982
    Illegal EcoRI site found at 3610
    Illegal EcoRI site found at 4162
    Illegal XbaI site found at 4168
    Illegal SpeI site found at 1107
    Illegal PstI site found at 4147
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2961


[edit]
Categories
Parameters
None